More than a dozen female athletes have sued the National Collegiate Athletic Association over its transgender participation policy, which the athletes claim violates their rights under Title IX, the law that prohibits discrimination based on sex at any institution that receives federal funding.

The lawsuit stems from the January 2022 ruling by the NCAA Board of Governors that allows transgender athletes to compete in the category of their affirmed gender on a sport-by-sport basis. The NCAA said that this ruling “preserves opportunity for transgender student-athletes while balancing fairness, inclusion and safety for all who compete.”

This week, 16 female athletes across sports like swimming, soccer and track and field, filed the lawsuit, saying in the lawsuit that they “bring this case to secure for future generations of women the promise of Title IX that is being denied them and other college women.”

“A lot of people ask us, why did we wait this long to file a lawsuit? Well, we waited this long to allow the NCAA every opportunity to make the right decision,” Kaitlynn Wheeler, a former University of Kentucky swimmer, told ABC News.

“The NCAA’s most basic job is to protect the fairness and the safety of its athletes, and it has failed on that simple task,” Wheeler said.

The complaint highlights an alleged incident at the 2022 NCAA national swimming championship — weeks after the NCAA’s ruling — involving former University of Kentucky swimmer Riley Gaines and Lia Thomas, a transgender swimmer who was competing for the University of Pennsylvania.

According to the lawsuit, Gaines had tied in fifth place with Thomas in the 200 free competition, but that as they prepared for the podium ceremony, Gaines was told by an NCAA official that only Thomas would be allowed to hold the fifth-place trophy.

Gaines says in the lawsuit she questioned the official, who the complaint alleges told her, “I’m so sorry, we have been advised that when photos are taken it is crucial that Lia Thomas holds the trophy.”

“The secret of Thomas’ meteoric ascendance and dominance in NCAA women’s swimming was retained male advantage,” the lawsuit alleges. Thomas had previously competed in the men’s category prior to the NCAA’s ruling.

“What the plaintiffs are really after is an equal and fair opportunity to succeed. For everybody to be equal at the starting line,” William Bock, the lead attorney in the lawsuit, told ABC News.

In a statement, the NCAA said that while it “does not comment on pending litigation, the Association and its members will continue to promote Title IX, make unprecedented investments in women’s sports and ensure fair competition in all NCAA championships.”

In 2022, Lia Thomas sat down with ABC News’ Juju Chang following the University of Pennsylvania swimmer’s historic Division 1 National title win.

“Trans people don’t transition for athletics. we transition to be happy and authentic and our true selves,” Thomas told Chang. “Transitioning to get an advantage is not something that ever factors into our decisions.”

“I knew there would be scrutiny against me if I competed as a woman. I was prepared for that,” Thomas said. “But I also don’t need anybody’s permission to be– myself and to– to do the sport that I love.”

Thomas did not immediately respond to ABC News’ requests for comment on the lawsuit.

“This lawsuit is the latest effort to eliminate the ability of transgender athletes to exist in the same spaces as cisgender athletes, and to erode the autonomy of sport governing bodies to set evidence-based eligibility criteria as they see fit,” Athlete Ally, a nonprofit LGBTQ athletic advocacy group, said in a new statement on Monday.

“We empathize with the pain felt by any athlete who falls short of their athletic goals, but singling out, scapegoating, or otherwise targeting trans athletes as the cause for those failures is unfounded and perpetuates stigma, stereotypes and discrimination against transgender Americans,” Hudson Taylor, founder and executive director of Athlete Ally, said.